| 产品英文名称 | Mouse Leptin Protein |
| 产品中文名称 | 重组小鼠瘦素蛋白 |
| 别名 | Leptin; Obese Protein; Obesity Factor; LEP; OB; OBS |
| 序列 | Recombinant Mouse Leptin is produced by our E.coli expression system and the target gene encoding Val22-Cys167 is expressed. |
| 活性 | Immobilized Recombinant Mouse LEPR (C-10His) at 5μg/ml (100 μl/well) can bind Recombinant Mouse Leptin: Biotinylated by NHS-biotin prior to testing.The ED50 of Recombinant Mouse Leptin is 1.16 ng/ml.(Regularly tested). |
| 蛋白长度 | 16.1 KDa |
| 纯度 | > 95% ，使用SDS-PAGE检测 |
| 特点&优势 | 采用 LAL 法检测，每 1 μg 蛋白质中的 EU 含量小于 1.0。 |
| 产品形式 | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, pH7.5. |
| 基因ID | 16846 |
| 蛋白序列链接 | https://www.uniprot.org/uniprotkb/P41160/entry |
| 使用中注意事项 | 开盖使用前，请先离心。本产品为冻干粉，推荐用该产品配套的Reconstitution Buffer进行复溶，且浓度不低于100 µg/mL。客户可根据后续的实验需求，进行进一步的稀释。 |
| 保存建议 | 本产品为冻干粉形式，建议将产品保存于-20℃以下的干燥环境中。本产品溶解之后，可在4℃保存2-7天，长期使用请置于-20℃以下。为长期保存，推荐添加载体蛋白（0.1% HSA或者BSA），并酌情分装保存，避免反复冻融。 |
| 运输条件 | 冰袋运输（蓝冰） |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
| 背景 | Leptin is a hormone secreted from white adipocytes and plays important role in the regulation of food intake and energy balance. Leptin functions via signaling pathways involving OB-R in hypothalamus. Animal models have revealed the influence of Leptin in reducing body weight and regulating blood glucose level. When mutations are introduced in obese gene, mice with impaired function of leptin are massively obese and in high risk of diabetes. Leptin deficiency reduces metablic rate. Leptin deficient mice are less active and with lower body temperature than normal animals. Human Leptin shares approximately 84% sequence identity with the mouse protein. Human Leptin consists of 167 amino acid residue including a 21 amino acid residue signal sequence and 146 amino acid residue mature protein sequence. |
Fig.重组小鼠瘦素蛋白
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