| 产品英文名称 | Mouse β-NGF protein |
| 产品中文名称 | 重组小鼠神经生长因子-β |
| 别名 | Beta-NGF; Beta-Nerve Growth Factor; NGF; NGFB |
| 标签 | 无标签 |
| 表达宿主 | 大肠杆菌 |
| 种属 | 小鼠 |
| 序列 | Amino acid sequence derived from Mouse β-NGF protein (P01139)(Met121—Gly241)was expressed. |
| 活性 | Measure by its ability to induce TF-1 cells proliferation. The ED50 for this effect is < 0.03ng/mL. The specific activity of recombinant Mouse β-NGF is > 3.33 x 107U/mg. |
| 蛋白长度 | The protein has a calculated MW of 14.41 kDa. |
| 纯度 | > 98% ，使用SDS-PAGE检测 |
| 特点&优势 | 采用 LAL 法检测，每 1 μg 蛋白质中的 EU 含量小于 0.1。 |
| 产品形式 | Lyophilized from a 0.2 µm filtered solution containing 20 mM sodium citrate, 0.15 M NaCl, pH 7.5. |
| 基因ID | 18049 |
| 蛋白序列链接 | https://www.uniprot.org/uniprotkb/P01139/entry |
| 使用中注意事项 | 开盖使用前，请先离心。本产品为冻干粉，推荐用该产品配套的Reconstitution Buffer进行复溶，且浓度不低于100 µg/mL。客户可根据后续的实验需求，进行进一步的稀释。 |
| 保存建议 | 本产品为冻干粉形式，建议将产品保存于-20℃以下的干燥环境中。本产品溶解之后，可在4℃保存2-7天，长期使用请置于-20℃以下。为长期保存，推荐添加载体蛋白（0.3% HSA或者BSA），并酌情分装保存，避免反复冻融。 |
| 运输条件 | 冰袋运输（蓝冰） |
| 警告 | 本文列出的产品仅供研究使用，不适用于人类或临床诊断。我们产品所推荐应用，不是建议使用我们的产品去违反任何专利或许可证。对于使用本产品可能发生的专利侵权或其他违规行为，我们不承担任何责任。 |
| 背景 | Nerve growth factor (NGF) influences the survival and differentiation of a variety of neuronal and nonneuronal cells. The actions of this polypeptide result from binding to specific cell surface receptors which are present as both high and low affinity sites. Although both types of receptor bind NGF only the high affinity form is capable of signal transduction. Cross-linking studies with 125I-NGF have shown that the high affinity receptor of Rat PC12 cells forms an Mr 158,000 complex with NGF while the low affinity receptor forms an Mr 100,000 complex. Full length cDNA clones for both the human and Rat NGF receptor genes have been produced and sequenced, revealing greater than 90% homology between the two species. |
Fig.重组小鼠神经生长因子-β
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