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Fig.KTB1600 - CheKine™ 还原型谷胱甘肽（GSH）检测试剂盒（微量法）

• 你好，请问测定动物组织的GSH，需要灌流去除血液吗，血液会不会对GSH有影响。另外，组织一般用多少g呢

  灌流去除血液较好，血液有影响，尽量去除。先按10毫克制备样品

• 细胞用反复冻融法处理的具体步骤是什么？

  放在-80冻15min，迅速37度水浴5-10min，短暂涡旋，裂解细胞，提醒使用耐冻的离心管；如果样本量较少，5min即可完成冻/融（实际操作1mL细胞悬液）；核心是速冻速融，缓慢冷冻会形成大冰晶，主要存在于细胞外，导致细胞脱水但不易破碎；缓慢融化会重结晶，进一步破坏细胞结构，但速冻速融总体效率更高。

• 请问动物组织加了蛋白去除试剂匀浆后可以测蛋白浓度吗？如果不可以该怎么测蛋白浓度呢

  加了蛋白去除试剂匀浆后的样本是不能做蛋白定量的，不准确的，如果要检测蛋白浓度要么平行准备两份样本一份加PBS或者其他合适的溶液匀浆，一份加蛋白去除试剂处理后进行GSH、GSSG的检测。

本产品已在(43)种出版物中被引用

• The miR-34a-5p promotes hippocampal neuronal ferroptosis in epilepsy by regulating SIRT1.

  作者：Gao, Fan, Zhenlin Yang, and Jinzi Li. 杂志名称： Neurochemical Research  IF：4

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• DCAF7 recruits USP2 to facilitate hepatocellular carcinoma progression by suppressing clockophagy-induced ferroptosis

  作者：H Jiang, X Wang, Z Zhu, C Song, D Li, Y Yun, ... 杂志名称：Animals (Basel) IF：10

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• Ponicidin Promotes Ferroptosis to Enhance Treatment Sensitivity in Lenvatinib-resistant Hepatocellular Carcinoma Cells through Regulation of KEAP1/NRF2.

  作者：Zhang, Lisha, et al. 杂志名称：Phytomedicine  IF：8

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• tRF3-IleAAT reduced extracellular matrix synthesis in diabetic kidney disease mice by targeting ZNF281 and inhibiting ferroptosis

  作者：乔云阳 杂志名称：Front Microbiol IF：8

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• SCARA5 induced ferroptosis to effect ESCC proliferation and metastasis by combining with Ferritin light chain

  作者：Y Liu, R Xiong, T Xiao, L Xiong, J Wu, J Li, G Feng, ... 杂志名称：Toxins IF：3

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• The role of MOTS-c-mediated antioxidant defense in aerobic exercise alleviating diabetic myocardial injury

  作者：M Tang, Q Su, Y Duan, Y Fu, M Liang, Y Pan, J Yuan, ... 杂志名称：Journal of medical virology IF：4

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• Evaluating Oxidative Stress, Nitric Oxide Production and Mitochondrial Activity Trend in Broiler Blood Cells under Progressive Cold Stress

  作者：S Juntautsa, K Pimpjong, ... 杂志名称：The EMBO Journal  IF：0

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• Ultrasmall MnOx nanodots catalyze glucose for reactive oxygen species‐dependent sequential anti‐infection and regeneration therapy.

  作者：Wang, Feng, et al. 杂志名称：Small Structures IF：14

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• Estradiol regulates MBOAT1‐mediated ferroptosis and participates in the progression of endometriosis

  作者：YQ Fang, YH Li, JY Li, XP Wang, ... 杂志名称：J Affect Disord IF：2

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• Recombinant human brain natriuretic peptide attenuates ischemic brain injury in mice by inhibiting oxidative stress and cell apoptosis via activation of PI3K/AKT/Nrf2 …

  作者：T Li, DJ Su, HW Lu, YQ Gao, YG Liu, SH Wang, ... 杂志名称：Drug Des Devel Ther IF：2

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• Umbilical cord mesenchymal stem cell-derived apoptotic extracellular vesicles ameliorate cutaneous wound healing in type 2 diabetic mice via macrophage …

  作者：Y Wang, L Jing, X Lei, Z Ma, B Li, Y Shi, ... 杂志名称：Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease  IF：7

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• Lactate and H3K18 Lactylation Contribute To the Exacerbation of Acute Pancreatitis by Modulating NCOA4-mediated Ferroptosis

  作者：M NCOA4-mediated 杂志名称：BioMed Research International, Volume 2019 IF：

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• Tumor Microenvironment-Activatable Metal-Phenolic Nanoformulations for Ultrasound-Boosted Ferroptosis through Triple Regulatory Pathways

  作者：Liu Zhendong刘振东 杂志名称：Nat Commun IF：18

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• Effects of a diet containing glutamine on the slaughter performance, meat quality, and skeletal muscle fiber types of feed-restricted yaks

  作者：Z Yue, S Ke, L Shi, R Hu, Z Wang, Q Peng, H Zou, ... 杂志名称：Nature Communications  IF：6

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• Cuproptosis improves CAR-T cell therapy in osteosarcoma

  作者：Z Huang, N Li, Z Gao, J Chen, Z Xue, C Wu, J Chen, ... 杂志名称：Respiratory Research IF：

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• Unlocking the potential of Traditional Chinese Medicine (TCM): Shipi Xiaoji formula (SPXJF) as a novel ferroptosis inducer in hepatocellular carcinoma.

  作者：Yang, Renyi, et al. 杂志名称： Journal of Ethnopharmacology  IF：5

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• Ursolic acid inhibits the proliferation of triple‑negative breast cancer stem‑like cells through NRF2‑mediated ferroptosis.

  作者：Yang, Xinhua, et al. 杂志名称：Oncology Reports IF：4

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• HO-1 suppression by co-culture-derived IL-6 alleviates ferritinophagy-dependent oxidative stress to potentiate myogenic differentiation

  作者：M Zhang, S Liu, Y Wang, S Shan, M Cang 杂志名称：Scientia Horticulturae IF：5

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• HIF-2α/HILPDA promotes ferroptosis sensitivity in placenta trophoblast cells of early-onset preeclampsia

  作者：Q Wang, N Yang, H Chen, B Ding, C Dou, X Wang, ... 杂志名称：Front Cell Dev Biol IF：2

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• Curcumin induces ferroptosis in hepatocellular carcinoma by regulating PERK/Nrf2/HO-1 signaling pathway

  作者：C Yuan, R Liu, X Xu, J Sun, Y Zhu, R Lu, ... 杂志名称： Biomedical and Environmental Sciences  IF：2

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• DMT1 ubiquitination by Nedd4 protects against ferroptosis after intracerebral hemorrhage.

  作者：Lv, Bingchen, et al. 杂志名称：CNS Neuroscience & Therapeutics IF：5

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• Polydatin attenuates cisplatin‐induced acute kidney injury by inhibiting ferroptosis

  作者：L Zhou, P Yu, T Wang, Y Du, Y Chen, ... 杂志名称：Science Advances  IF：7

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• Ultrasound Trigger Ultrasmall Multifunction Nanobubbles for Thrombus Visual Treatment

  作者：马子珺 杂志名称：Human Genetics IF：10

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• MicroRNA‐424‐5p Alleviates Isoflurane Anesthesia‐Induced Neurotoxicity in Human Embryonic Stem Cell‐Derived Neurons by Targeting FASN

  作者：X Gu, W Yue, M Xiu, Q Zhang, ... 杂志名称：EBioMedicine IF：3

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• Chinese ginger (Boesenbergia rotunda) mitigates diabetic nephropathy and cardiomyopathy by regulating oxidative inflammatory pathway

  作者：C Liu, X Tang, M Zhang, Y Wu, A Famurewa, ... 杂志名称：International Immunopharmacology IF：2

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• Fe（III）-juglone nanoscale coordination polymers for cascade chemodynamic therapy through synergistic ferroptosis and apoptosis strategy

  作者：Zhendong Liu 杂志名称：International Journal of Molecular Medicine IF：9

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• ZIF-8-based nanoparticles for inflammation treatment and oxidative stress reduction in periodontitis.

  作者：Li, Yaxin, et al. 杂志名称：ACS Applied Materials & Interfaces IF：8

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• Modulation of liver metabolism and gut microbiota by Alhagi-honey alleviated heat stress-induced liver damage.

  作者：Xu, Jing, et al. 杂志名称：Stress Biology IF：3

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• Comprehensive Profiling of Transcriptome and m6A Epitranscriptome Uncovers the Neurotoxic Effects of Yunaconitine on HT22 Cells.

  作者：Lin, Beian, et al. 杂志名称：Evolutionary Bioinformatics IF：2

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• Biphasic protective effects of macrophage migration inhibitory factor in ischemia/reperfusion-induced acute kidney injury

  作者：Y Du, C Feng, L Zhou, T Wang, H Ma, H Liu 杂志名称：Chen D Q , Chen L, et al IF：5

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• Dietary Glutamine Supplementation Alleviated Rumen Epithelium Oxidative Damage and Apoptosis Induced by Feed Restriction via Maintaining Mitochondrial …

  作者：Z Yue, S Ke, L Shi, R Hu, Z Wang, ... 杂志名称：Plant Biotechnology Journal IF：

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• ZNF526 drives tumor growth by enhancing SHMT1-dependent serine metabolism and antioxidant capability in TNBC

  作者：W Qin, L Shao, Q Li, D Zhang, X Jia, C Dong 杂志名称：Microorganisms IF：34

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• Quercetin Attenuates Acute Kidney Injury Caused by Cisplatin by Inhibiting Ferroptosis and Cuproptosis.

  作者：Shi, Mengqi, Youchaou Mobet, and Hong Shen. " 杂志名称：Cell Biochemistry and Biophysics IF：2

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• Polystyrene nanoplastics with different functional groups and charges have different impacts on type 2 diabetes.

  作者：Wang, Yunyi, et al. 杂志名称：Particle and Fibre Toxicology IF：7

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• The dual angiogenesis effects via Nrf2/HO-1 signaling pathway of melatonin nanocomposite scaffold on promoting diabetic bone defect repair.

  作者：Chen, Tingting, et al. 杂志名称：International journal of nanomedicine IF：7

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• Glutamine relieves feed restriction-induced ruminal epithelial function damage through histone lysine lactylation in yaks

  作者：Z Yue, L Shi, Z Wang, R Hu, Q Peng, H Zou, ... 杂志名称：Reprod Sci IF：6

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• A multimodal MR imaging study of the effect of hippocampal damage on affective and cognitive functions in a rat model of chronic exposure to a plateau …

  作者：D Zhu, B He, M Zhang, Y Wan, R Liu, L Wang, ... 杂志名称：Molecular immunology IF：4

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• Regulation of macrophage plasticity by circCCDC719-13 through HSP90 inhibition suppresses prostate cancer progression and metastasis: a translational study.

  作者：Cheng, Bisheng, et al. 杂志名称：International Journal of Surgery IF：10

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  作者：CC PANSA 杂志名称：FASEB Journal IF：

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• Osteoarthritis pathogenesis Unveiled: DMT1 drives autophagy-dependent ferroptosis through a mir-17-5p/NEDD4 regulatory axis

  作者：G Zhao, Y Shen, J Ma, X Huang, J Chen, J Shi, ... 杂志名称：Respiratory Research  IF：8

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• The role of sex and ovarian hormones in hippocampal damage and cognitive deficits induced by chronic exposure to hypobaric hypoxia

  作者：D Zhu, M Zhang, B He, Y Wan, L Wang, ... 杂志名称：Journal of Biological Chemistry IF：3

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• Potential Action Mechanism of Erianin in Relieving MNNG-triggered Chronic Atrophic Gastritis.

  作者：Jiang, Qianqian, Guoxia Fan, and Kaiwei Wu. 杂志名称：Cell Biochemistry and Biophysics IF：2

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• Intracellular C5aR1 inhibits ferroptosis in glioblastoma through METTL3-dependent m6A methylation of GPX4.

  作者：Meng, Xiangrui, et al. 杂志名称：Cell Death & Disease IF：8

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