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Fig.KTB1110 - CheKine™ 乳酸脱氢酶（LDH）活性检测试剂盒（微量法）

• 检测细胞培养液时，怎么设置对照组消除血清干扰

  在检测细胞培养液中的乳酸脱氢酶时，由于血清含有乳酸脱氢酶，使用含血清的培养液会增加背景读数，在检测时一定要设置没有细胞，但加入了相同体积培养液的对照孔，以用于消除背景。血清含量越高，背景值越高。如果对于实验无明显影响，建议使用灭活血清，这样血清中的乳酸脱氢酶会被很大程度上失活，大幅降低背景。如果对于实验无明显影响，实验时可以使用无血清培养液或血清浓度较低的培养液，这样能有效降低血清中乳酸脱氢酶的本底活性。

本产品已在(17)种出版物中被引用

• The Bcr-Abl inhibitor DCC-2036 inhibits necroptosis and ameliorates osteoarthritis by targeting RIPK1 and RIPK3 kinases

  作者：L Piao, D Wu, C Rui, Y Yang, S Liu, J Liu, Z Jin, ... 杂志名称：Front. Pharmacol IF：8

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• A Comparative Study on Low and High Salinity Tolerance of Two Strains of Pinctada fucata

  作者：J Sun, M Chen, Z Fu, J Yang, S Zhou, G Yu, ... 杂志名称：Brain Research Bulletin IF：4

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• Apabetalone, a BET protein inhibitor, inhibits kidney damage in diabetes by preventing pyroptosis via modulating the P300/H3K27ac/PLK1 axis.

  作者：Wang, Min, et al. 杂志名称：Pharmacological Research IF：9

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• Calcium-mediated mitochondrial fission and mitophagy drive glycolysis to facilitate arterivirus proliferation

  作者：Z Sun, Z Ma, W Cao, C Jiang, L Guo, K Liu, ... 杂志名称：Autophagy IF：5

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• A Novel Prognostic Signature Integrating Immune and Glycolytic Pathways for Enhanced Prognosis and Immunotherapy Prediction in Hepatocellular Carcinoma

  作者：Z Zhang, H Zhao, P Wang, X Geng, M Yin, ... 杂志名称：Cells IF：3

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• Tryptophan deficiency induced by indoleamine 2, 3‐dioxygenase 1 results in glucose transporter 1‐dependent promotion of aerobic glycolysis in pancreatic cancer.

  作者：Liang, Heng, et al. 杂志名称：MedComm IF：11

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• Canagliflozin ameliorates NLRP3 inflammasome-mediated inflammation through inhibiting NF-κB signaling and upregulating Bif-1

  作者：Y Niu, Y Zhang, W Zhang, J Lu, Y Chen, ... 杂志名称：Neuromolecular Med IF：4

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• Targeting HDAC6 attenuates nicotine-induced macrophage pyroptosis via NF-κB/NLRP3 pathway

  作者：S Xu, H Chen, H Ni, Q Dai 杂志名称：Front Oncol IF：6

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• Physical Responses of Pinctada fucata to Salinity Stress

  作者：J Yang, J Yang, M Chen, Z Fu, J Sun, G Yu, ... 杂志名称：Neuroscience IF：3

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• Spermidine mitigates lipopolysaccharide-induced acute lung injury by enhancing autophagic flux

  作者：X Zhang, H Lu, X Xu 杂志名称：Biomed Pharmacother IF：2

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• Mitochondrial CISD1 Modulates Microglial Metabolic Reprogramming to Drive Stress Susceptibility in Mice

  作者：W Dong, D Liu, S Fu, J Zhang, X Chen, ... 杂志名称：Nature Communications IF：14

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• A high-throughput Gaussia luciferase reporter assay for screening potential gasdermin E activators against pancreatic cancer

  作者：Y Liu, X Zhang, P Zhang, T He, W Zhang, D Ma, ... 杂志名称：Fish Physiol Biochem IF：15

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• Spreading of P301S Aggregated Tau Investigated in Organotypic Mouse Brain Slice Cultures

  作者：DS Korde, C Humpel 杂志名称：EBioMedicine IF：5

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• Circadian disruption by simulated shift work aggravates periodontitis via orchestrating BMAL1 and GSDMD-mediated pyroptosis

  作者：Y Wang, R Li, Q Ye, D Fei, X Zhang, J Huang, ... 杂志名称：Journal of Ethnopharmacology IF：5

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• TAB182 regulates glycolytic metabolism by controlling LDHA transcription to impact tumor radiosensitivity.

  作者：Chen, Shi, et al. 杂志名称：Cell Death & Disease IF：8

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• Metabolic and immunomodulatory control of type 2 diabetes via generating cellular itaconate reservoirs by inflammatory-targeting gene-therapy nanovesicles

  作者：A Lao, W Li, Y Sun, Y Cao, Y Zhuang, J Wu, D Li, ... 杂志名称：Nanoscale Res Lett IF：15

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• Binary pyroptosis-amplified self-assembling prodrug nanomedicine enhances immunogenicity and inhibits abdominal metastasis in ovarian cancer

  作者：方锋 杂志名称：Frontiers in Psychiatry IF：15

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